Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light and Immunogold Electron Microscopy in Drug Discovery and Safety Assessment Research
نویسندگان
چکیده
Freeze Substitution and Low Temperature Processing of Cryo Protected Tissues into Lowicryl HM23 Resin for Correlated Light Immunogold Electron Microscopy in Drug Discovery Safety Assessment Research - Volume 28 Issue S1
منابع مشابه
Lowicryl K4M embedding of brain tissue for immunogold electron microscopy.
We present methods for embedding brain tissue in Lowicryl K4M embedding medium and localizing antigens using postembedding immunogold techniques. After perfusion fixation with 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M cacodylate buffer, blocks of rat brain were placed in 2% aqueous uranyl acetate for 1 hour, dehydrated in 50%, 70%, and 95% ethanol, infiltrated with Lowicryl/ethanol m...
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15 صفحه اولLow-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
The coupling of cryo-light microscopy (cryo-LM) and cryo-electron microscopy (cryo-EM) poses a number of advantages for understanding cellular dynamics and ultrastructure. First, cells can be imaged in a near native environment for both techniques. Second, due to the vitrification process, samples are preserved by rapid physical immobilization rather than slow chemical fixation. Third, imaging ...
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although there are studies on pragmatic assessment, to date, literature has been almost silent about native and non-native english raters’ criteria for the assessment of efl learners’ pragmatic performance. focusing on this topic, this study pursued four purposes. the first one was to find criteria for rating the speech acts of apology and refusal in l2 by native and non-native english teachers...
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Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) were first realized in the early 1980s [1] and mid-1990s [2, 3]. Since then both methods have quickly become a powerful tools in structural biology. The major advantage is that they allow for the acquisition of images of biological specimens persevered in their native state after they are plunge-frozen onto carbon-coated ...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2022
ISSN: ['1435-8115', '1431-9276']
DOI: https://doi.org/10.1017/s1431927622005670